Article
Identification of defects in glycosylphosphatidylinositol anchor biosynthesis in the Thy-1 expression mutants.
The Journal of biological chemistry - 5 Jul 1991
Sugiyama E, DeGasperi R, Urakaze M, Chang H M, Thomas L J, Hyman R, Warren C D, Yeh E T
Abstract excerpt
A number of eukaryotic proteins are anchored to the membrane by glycosylphosphatidylinositol (GPI), of which the core structure is conserved from protozoan to mammalian cells. Here, we used a panel of thymoma mutants, which synthesize Thy-1 but cannot express it on the cell surface, to study the GPI biosynthetic pathway in mammalian cells. These mutants have been assigned into six complementation classes (A, B,...
Topics
- Autoradiography
- Cell Line
- Cell-Free System
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Glycolipids
- Glycosylphosphatidylinositols
- Membrane Glycoproteins
- Mutation
- Phosphatidylinositols