Article
Multilocus sequence typing of oenological Saccharomyces cerevisiae strains.
Food microbiology - 1 Dec 2009
Muñoz Rosario, Gómez Alicia, Robles Virginia, Rodríguez Patricia, Cebollero Eduardo, Tabera Laura, Carrascosa Alfonso V, Gonzalez Ramon
Abstract excerpt
This study describes the application of a multilocus sequence typing (MLST) analysis for molecular discrimination at the strain level of Spanish wine yeast strains. The discrimination power of MLST is compared to mitochondrial RFLP analysis. Fragments of the ADP1, ACC1, RPN2, GLN4, and ALA1 genes were amplified by PCR from chromosomal DNA of 18 wine Saccharomyces cerevisiae strains. Ten polymorphic sites were...
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