Article
Enrichment of sequencing targets from the human genome by solution hybridization.
Genome biology - 1 Jan 2009
Tewhey Ryan, Nakano Masakazu, Wang Xiaoyun, Pabón-Peña Carlos, Novak Barbara, Giuffre Angelica, Lin Eric, Happe Scott, Roberts Doug N, LeProust Emily M, Topol Eric J, Harismendy Olivier, Frazer Kelly A
Abstract excerpt
To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligonucleotide capture probes in solution to enrich for approximately 3.9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage...
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