Article
Translocation and endocytosis for cell-penetrating peptide internalization.
The Journal of biological chemistry - 4 Dec 2009
Jiao Chen-Yu, Delaroche Diane, Burlina Fabienne, Alves Isabel D, Chassaing Gérard, Sagan Sandrine
Abstract excerpt
Cell-penetrating peptides (CPPs) share the property of cellular internalization. The question of how these peptides reach the cytoplasm of cells is still widely debated. Herein, we have used a mass spectrometry-based method that enables quantification of internalized and membrane-bound peptides. Internalization of the most used CPP was studied at 37 degrees C (endocytosis and translocation) and 4 degrees C...
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