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Article

Circular permutation of ligand-binding module improves dynamic range of genetically encoded FRET-based nanosensor.

Protein science : a publication of the Protein Society - 1 Dec 2009

Okada Satoshi, Ota Kazuhisa, Ito Takashi

Abstract excerpt

Quantitative measurement of small molecules with high spatiotemporal resolution provides a solid basis for correct understanding and accurate modeling of metabolic regulation. A promising approach toward this goal is the FLIP (fluorescent indicator protein) nanosensor based on bacterial periplasmic binding proteins (PBPs) and fluorescence resonance energy transfer (FRET) between the yellow and cyan variants of...

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Identifiers

PMID: 19827096
DOI: 10.1002/pro.266
PMCID: PMC2821270

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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