Engineering a circularly permuted GFP scaffold for peptide presentation.

Abstract excerpt

The use of peptides as in vivo and in vitro ligand binding agents is hampered by the high flexibility, low stability and lack of intrinsic detection signal of peptide aptamers. Recent attempts to overcome these limitations included the integration of the binding peptide into a stable protein scaffold. In this paper, we present the optimization and testing of a circularly permuted variant of the green fluorescent...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Guanidine
• Share to Peptide Library
• Share to Peptides