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Article

Creation of circularly permutated yellow fluorescent proteins using fluorescence screening and a tandem fusion template.

Biotechnology letters - 1 Apr 2006

Chiang Jason Jui-Hsuan, Li Isaac, Truong Kevin

Abstract excerpt

By experimenting with many different circularly permutated yellow fluorescent protein (cpYFP) variants as acceptors in fluorescence resonance energy transfer based biosensors, the optimal dynamic range can be discovered by sampling the possibilities of relative fluorophore orientations before and after bioactivity. Hence, to facilitate the sampling process, we introduced a new approach to construct a library of...

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PMID: 16614928
DOI: 10.1007/s10529-006-0007-6

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