Article

Adaptable, Turn-On Monobody (ATOM) Fluorescent Biosensors for Multiplexed Detection in Cells

2023-03-28

Abstract excerpt

A grand challenge in biosensor design is to develop a single molecule, fluorescent protein-based platform that can be easily adapted to recognize targets of choice. Conceptually, this can be achieved by fusing a small, antibody-like binding domain to a fluorescent protein in such a way that target binding activates fluorescence. Although this design is simple to envision, its execution is not obvious. Here, we cre...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 98fe639f-4836-58e0-8cfb-c1fbd94dedd1
DOI: 10.1101/2023.03.28.534597

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Adaptable, Turn-On Monobody (ATOM) Fluorescent Biosensors for Multiplexed Detection in CellsDOI 10.1101/2023.03.28.534597Read

Creating Red Light-Controlled Protein Dimerization Systems as Genetically Encoded Actuators with High SpecificitySemantic proximity Read

Differential regulation of protein subdomain activity with caged bivalent ligands.Semantic proximity Read

Detection and manipulation of live antigen-expressing cells using conditionally stable nanobodiesSemantic proximity Read

Triple labeling resolves a GPCR intermediate state by 3-color single molecule FRETSemantic proximity Read

Bioluminescent detection of viral surface proteins using branched multivalent protein switchesSemantic proximity Read

Principles and applications of green fluorescent protein-based biosensors: a mini-review.Semantic proximity Read

Fluorescent proteins and their applications in imaging living cells and tissues.Semantic proximity Read

Improved Chemical-Genetic Fluorescent Markers for Live Cell Microscopy.Semantic proximity Read

Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell MappingSemantic proximity Read

Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.Semantic proximity Read

Evaluation and Selection of Potent Fluorescent Immunosensors by Combining Fluorescent Peptide and Nanobodies Displayed on Yeast SurfaceSemantic proximity Read

Engineering modular intracellular protein sensor-actuator devicesSemantic proximity Read