HLA class II typing by digestion of PCR-amplified DNA with allele-specific restriction endonucleases will fail to unequivocally identify the genotypes of many homozygous and heterozygous individuals.

Abstract excerpt

Recently, a new technique for HLA class II genotyping has been introduced, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, claimed to be a practical alternative to conventional serological and cellular HLA class II typing (1-3). The PCR-RFLP technique is...

Topics

• Alleles
• DNA Probes, HLA
• DNA Restriction Enzymes
• Genotype
• HLA-D Antigens
• HLA-DP Antigens
• HLA-DQ Antigens
• HLA-DR Antigens
• Heterozygote
• Histocompatibility Testing
• Homozygote
• Humans
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Reproducibility of Results
• Substrate Specificity