Article
PCR-RFLP typing detects new HLA-DRB1 alleles: DRB1*13022, DRB1*1336 and DRB1*1435.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Aug 2001
Tréjaut J, Kennedy A, Hobart D, Le T, Greville W D, Ng G, Taverniti A, Dunckley H
Abstract excerpt
It is difficult to resolve all heterozygous combinations of the HLA-DRB1*03, *08, *11, *12, *13 and *14 allele group in a one-step generic HLA-DRB1 typing system. Therefore, it is common to employ a secondary technique utilizing group-specific primers to amplify this group of alleles separately from the other HLA-DRB1, -DRB3, -DRB4 and -DRB5 alleles. This paper describes a polymerase chain reaction-restriction...
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