Article
HLA class II genotyping by polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP) in systemic lupus erythematosus patients.
Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology - 1 Jun 1995
Xavier R M, Ishikura H, Iwata Y, Tsumura H, Kobayashi S, Endo J
Abstract excerpt
Recently, genotyping of HLA class II alleles by digestion of polymerase chain reaction (PCR) amplified polymorphic DNA region with restriction endonucleases (PCR-RFLP) has been reported and claimed to be a simple and reliable technique. We tested the use of PCR-RFLP for genotyping of DQA 1, DQB 1, DPB 1 and DRB 1 alleles of 40 normal individuals and 19 patients with systemic lupus erythematosus (SLE). In...
Topics
- Genotype
- Histocompatibility Antigens Class II
- Humans
- Lupus Erythematosus, Systemic
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length