Article
pHAZE: a shuttle vector system for the detection and analysis of ionizing radiation-induced mutations.
Mutation research - 1 Dec 1990
Lutze L H, Winegar R A
Abstract excerpt
We have designed and constructed a shuttle vector system, pHAZE, that is maintained as an episome in normal human fibroblasts, has a low background mutation frequency, and is capable of detecting a spectrum of mutations, including deletions up to 8.3 kb. The efficacy of this system was demonstrat...
Topics
- Cell Line
- Chromosome Deletion
- Clone Cells
- DNA
- Genetic Vectors
- Humans
- Mutation
- Plasmids
- Polymorphism, Restriction Fragment Length