Article
Spectra and characteristics of somatic mutations induced by ionizing radiation in hematopoietic stem cells.
Proceedings of the National Academy of Sciences of the United States of America - 11 Apr 2023
Matsuda Yukiko, Uchimura Arikuni, Satoh Yasunari, Kato Naohiro, Toshishige Masaaki, Kajimura Junko, Hamasaki Kanya, Yoshida Kengo, Hayashi Tomonori, Noda Asao, Tanabe Osamu
Abstract excerpt
Spectra and frequencies of spontaneous and X-ray-induced somatic mutations were revealed with mouse long-term hematopoietic stem cells (LT-HSCs) by whole-genome sequencing of clonal cell populations propagated in vitro from single isolated LT-HSCs. SNVs and small indels were the most common types of somatic mutations, and increased up to twofold to threefold by whole-body X-irradiation. Base substitution patterns...
Topics
- Animals
- Mice
- Radiation, Ionizing
- Mutation
- Mutagenesis
- X-Rays
- Hematopoietic Stem Cells