Temperature-sensitive mutant of Bacillus subtilis glutamine synthetase obtained by random mutation.

doi:10.1093/oxfordjournals.jbchem.a123149

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Article

Temperature-sensitive mutant of Bacillus subtilis glutamine synthetase obtained by random mutation.

Journal of biochemistry - 1 Jul 1990

Nakano Y, Kimura K

Abstract excerpt

Random mutations were introduced into the B. subtilis glutamine synthetase gene by using nitrous acid, and a high temperature-sensitive mutant was selected. DNA sequencing of the restriction fragment containing the mutation revealed a single base-pair change resulting in the substitution of Leu 3...

Topics

Bacillus subtilis
Base Sequence
Chymotrypsin
Circular Dichroism
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Glutamate-Ammonia Ligase
Kinetics
Molecular Sequence Data
Mutation
Temperature

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