Article
The transcriptional activator NtrC controls the expression and activity of glutamine synthetase in Herbaspirillum seropedicae.
FEMS microbiology letters - 15 Nov 2000
Persuhn D C, Souza E M, Steffens M B, Pedrosa F O, Yates M G, Rigo L U
Abstract excerpt
The role of the Ntr system in Herbaspirillum seropedicae was determined via ntrB and ntrC mutants. Three phenotypes were identified in these mutants: Nif(-), deficiency in growth using nitrate, and low glutamine synthetase (GS) activity. All phenotypes were restored by the plasmid pKRT1 containing the intact glnA, ntrB and ntrC genes of H. seropedicae. The promoter region of glnA was subcloned into a...
Topics
- Bacterial Proteins
- DNA-Binding Proteins
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Genetic Vectors
- Glutamate-Ammonia Ligase
- Gram-Negative Bacteria
- Mutation
- Nitrogen Fixation
- PII Nitrogen Regulatory Proteins
- Trans-Activators
- Transcription Factors
- beta-Galactosidase