Article
Isolation and characterization of a Neurospora crassa mutant altered in the alpha polypeptide of glutamine synthetase.
Journal of bacteriology - 1 Sept 1990
Calderón J, Martínez L M, Mora J
Abstract excerpt
We report the isolation and characterization of a Neurospora crassa glutamine synthetase (GS) mutant altered in one of the two polypeptides (GS alpha) of this enzyme. We used the gln-1bR8 mutant strain that synthesizes only the GS alpha monomer and lacks the GS beta monomer and selected for growth in minimal medium in the presence of alpha-methyl-DL-methionine-SR-sulfoximine (alpha-me-MSO), an inhibitor of GS...
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