Article
Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases.
Scientific reports - 21 May 2018
Li Xu, Zhang Xian, Xu Shuqin, Zhang Hengwei, Xu Meijuan, Yang Taowei, Wang Li, Qian Haifeng, Zhang Huiling, Fang Haitian, Osire Tolbert, Rao Zhiming, Yang Shangtian
Abstract excerpt
L-asparaginase, which catalyses the hydrolysis of L-asparagine to L-aspartate, has attracted the attention of researchers due to its expanded applications in medicine and the food industry. In this study, a novel thermostable L-asparaginase from Pyrococcus yayanosii CH1 was cloned and over-expressed in Bacillus subtilis 168. To obtain thermostable L-asparaginase mutants with higher activity, a robust...
Topics
- Asparagine
- Computational Biology
- Enzyme Stability
- High-Throughput Screening Assays
- Hot Temperature
- Mutagenesis, Site-Directed
- Mutant Proteins
- Mutation
- Protein Conformation
- Pyrococcus