Article
Characterization of NS3 protease from an Egyptian HCV genotype 4a isolate.
Archives of virology - 1 Jan 2009
Bahgat Mahmoud Mohamed, Ibrahim Ahmed Atef, Abd-Elshafy Dina Nadeem, Mesalam Ahmed Atef, Gewaid Hossam Eid, Ismaeil Amany Abd-Elghany, El-Waseef Ahmed Mohamed, Maghraby Amany Sayed, Barakat Ahmed Barakat, El-Far Mohamed Abd-Elhafez, Ghanem Hossam El-Din Ahmed, Mohamed Amro Mahmoud, Ali Mohamed Ahmed
Abstract excerpt
The role of the NS3 protease in HCV replication was demonstrated by the ability of a protease inhibitor cocktail (10 microg/ml) to abolish the induced cytopathic effect in RAW macrophages upon infection with Egyptian sera. The HCV protease gene was amplified from Egyptian sera by nested PCR and cloned downstream of the CMV promotor in a mammalian expression plasmid, which was then used to transform bacteria....
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