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Article

Establishment of a cell-based assay system for hepatitis C virus serine protease and its primary applications.

World journal of gastroenterology - 1 Nov 2003

Mao Hong-Xia, Lan Shui-Yun, Hu Yun-Wen, Xiang Li, Yuan Zheng-Hong

Abstract excerpt

AIM: To establish an efficient, sensitive, cell-based assay system for NS3 serine protease in an effort to study further the property of hepatitis C virus (HCV) and develop new antiviral agents. METHODS: We constructed pCI-neo-NS3/4A-SEAP chimeric plasmid, in which the secreted alkaline phosphatase (SEAP) was fused in-frame to the downstream of NS4A/4B cleavage site. The protease activity of NS3 was reflected by...

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Identifiers

PMID: 14606079
DOI: 10.3748/wjg.v9.i11.2474
PMCID: PMC4656523

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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