Dissection of nucleophilic and acid-base catalysis in glycosidases.

doi:10.1016/s1367-5931(01)00260-5

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Article

Dissection of nucleophilic and acid-base catalysis in glycosidases.

Current opinion in chemical biology - 1 Dec 2001

Zechel D L, Withers S G

Abstract excerpt

A startling array of added anions have been observed to function as replacement catalytic nucleophiles in mutant glycosidases, including formate, azide, fluoride and other halides. Likewise, the mechanism of acid-base catalysis is somewhat plastic. The carboxylic acids can be substituted by a sulfenic acid or by ascorbate, and the effective acid strength enhanced by the introduction of strong hydrogen bonds....

Topics

Azides
Carboxylic Acids
Catalysis
Fluorides
Formates
Glycoside Hydrolases
Muramidase
Mutation
Xylan Endo-1,3-beta-Xylosidase
Xylosidases

Source

Open in PubMed

Identifiers

PMID: 11738173
DOI: 10.1016/s1367-5931(01)00260-5

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