Article
The NEXT-A (N-terminal EXtension with Transferase and ARS) reaction.
Nucleic acids symposium series (2004) - 1 Jan 2009
Taki Masumi, Kuroiwa Hiroyuki, Sisido Masahiko
Abstract excerpt
L/F-transferase is known to catalyze transfer of hydrophobic amino acids from aminoacyl tRNA to the N-terminus of a protein possessing lysine or arginine as the N-terminus. Combining L/F-transferase with E. coli phenylalanyl-tRNA synthetase (ARS), we achieved non-ribosomal N-terminal-specific introduction of various kinds of nonnatural amino acids to a protein. A nonnatural amino acid is once charged onto an E....
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