Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.

Molecular bioSystems - 1 Sept 2009

Takimoto Jeffrey K, Adams Katrina L, Xiang Zheng, Wang Lei

Abstract excerpt

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acids
Share to Amino Acyl-tRNA Synthetases
Share to Azides

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cross-Linking Reagents

Share to Genes, Bacterial

Share to HeLa Cells

Share to Phenylalanine

Share to Transfection

Source

Identifiers

PMID: 19668857
DOI: 10.1039/b904228h

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells.PMID 19668857Read ↗

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code.Semantic proximity Read ↗

Genetic code expansion in mammalian cellsSemantic proximity Read ↗

Site-specific incorporation of a fluorescent terphenyl unnatural amino acid.Semantic proximity Read ↗

Directed evolution of aminoacyl-tRNA synthetases through in vivo hypermutation.Semantic proximity Read ↗

A chemical toolkit for proteins--an expanded genetic code.Semantic proximity Read ↗

Expanding the genetic code.Semantic proximity Read ↗

An expanded genetic code in mammalian cells with a functional quadruplet codon.Semantic proximity Read ↗

Multiple-site labeling of proteins with unnatural amino acids.Semantic proximity Read ↗

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system.Semantic proximity Read ↗

An Orthogonal Tyrosyl-tRNA Synthetase/tRNA Pair from a Thermophilic Bacterium for an Expanded Eukaryotic Genetic Code.Semantic proximity Read ↗

Five-base codons for incorporation of nonnatural amino acids into proteins.Semantic proximity Read ↗

Incorporation of non-canonical amino acids into proteins in yeast.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.