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Article

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 2004

Zhang Zhiwen, Alfonta Lital, Tian Feng, Bursulaya Badry, Uryu Sean, King David S, Schultz Peter G

Abstract excerpt

An orthogonal tryptophanyl-transfer RNA (tRNA) synthetase (TrpRS)-mutant opal suppressor tRNA(Trp) (mutRNA(UCA)(Trp)) pair was generated for use in mammalian cells. The anticodon loop of the Bacillus subtilis tRNA(Trp) was mutated to UCA, three positions in the D arm were mutated to generate an internal promoter sequence, and the mutRNA(UCA)(Trp) gene was inserted between the 5' and 3' flanking sequences of the...

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Identifiers

PMID: 15187228
DOI: 10.1073/pnas.0307029101
PMCID: PMC428441

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Source receipt: pubmed, retrieved 7 Sept 2026.

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