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Article

Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA.

The FEBS journal - 1 Oct 2009

Yang Zheng, Carey James F, Champoux James J

Abstract excerpt

Human topoisomerase I binds DNA in a topology-dependent fashion with a strong preference for supercoiled DNAs of either sign over relaxed circular DNA. One hypothesis to account for this preference is that a second DNA-binding site exists on the enzyme that mediates an association with the nodes present in supercoiled DNA. The failure of the enzyme to dimerize, even in the presence of DNA, appears to rule out the...

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Share to DNA, Superhelical
Share to DNA Topoisomerases, Type I
Share to Electrophoretic Mobility Shift Assay

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Nucleic Acid Conformation

Share to Protein Binding

Share to Protein Multimerization

Share to Protein Structure, Secondary

Source

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Identifiers

PMID: 19740104
DOI: 10.1111/j.1742-4658.2009.07270.x
PMCID: PMC3107988

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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