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Article

Directed evolution to increase camptothecin sensitivity of human DNA topoisomerase I.

Chemistry & biology - 1 Sept 2001

Scaldaferro S, Tinelli S, Borgnetto M E, Azzini A, Capranico G

Abstract excerpt

BACKGROUND: Human DNA topoisomerase I (top1) relaxes DNA supercoiling during basic nuclear processes. The enzyme is the main target of antitumor agents, such as camptothecins (CPT), that transform top1 into a DNA-damaging agent. RESULTS: By directed evolution of a C-terminal portion, we selected human top1 mutants that were 22-28-fold more CPT-sensitive than wild-type top1 in Saccharomyces cerevisiae cells. The...

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Identifiers

PMID: 11564555
DOI: 10.1016/s1074-5521(01)00059-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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