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Article

Characterization of recombinant human DNA topoisomerase IIIalpha activity expressed in yeast.

Journal of biochemistry - 1 Jun 2000

Hotoda N, Hanai R

Abstract excerpt

Recombinant human DNA topoisomerase IIIalpha was expressed in mutant yeast cells devoid of both topoisomerases I and III, and the gene product was partially purified. The activity of the protein in supercoil removal was found to be limited and also biphasic: in the first phase it processively changed the linking-number of hypernegatively supercoiled DNA, but only to the superhelicity of a regular negative...

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Identifiers

PMID: 10833282
DOI: 10.1093/oxfordjournals.jbchem.a022705

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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