Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Tryptophane-205 of human topoisomerase I is essential for camptothecin inhibition of negative but not positive supercoil removal.

Nucleic acids research - 1 Jan 2007

Frøhlich Rikke From, Veigaard Christopher, Andersen Félicie Faucon, McClendon A Kathleen, Gentry Amanda C, Andersen Anni Hangaard, Osheroff Neil, Stevnsner Tinna, Knudsen Birgitta Ruth

Abstract excerpt

Positive supercoils are introduced in cellular DNA in front of and negative supercoils behind tracking polymerases. Since DNA purified from cells is normally under-wound, most studies addressing the relaxation activity of topoisomerase I have utilized negatively supercoiled plasmids. The present...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Camptothecin
Share to DNA, Superhelical

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Topoisomerases, Type I

Share to Enzyme Inhibitors

Share to Kinetics

Share to Mutation

Share to Plasmids

Share to Tryptophan

Source

Open in PubMed Open full text

Identifiers

PMID: 17827209
DOI: 10.1093/nar/gkm669
PMCID: PMC2094083

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-17827209-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Tryptophane-205 of human topoisomerase I is essential for camptothecin inhibition of negative but not positive supercoil removal.PMID 17827209Read ↗

Regions within the N-terminal domain of human topoisomerase I exert important functions during strand rotation and DNA binding.Semantic proximity Read ↗

Control of RNA polymerase II promoter-proximal pausing by DNA supercoilingSemantic proximity Read ↗

Role of a tryptophan anchor in human topoisomerase I structure, function and inhibition.Semantic proximity Read ↗

Using energy to go downhill—a genoprotective role for ATPase activity in DNA topoisomerase IISemantic proximity Read ↗

Using energy to go downhill – a genoprotective role for ATPase activity in DNA topoisomerase IISemantic proximity Read ↗

Effect on DNA relaxation of the single Thr718Ala mutation in human topoisomerase I: a functional and molecular dynamics study.Semantic proximity Read ↗

Directed evolution to increase camptothecin sensitivity of human DNA topoisomerase I.Semantic proximity Read ↗

Characterization of recombinant human DNA topoisomerase IIIalpha activity expressed in yeast.Semantic proximity Read ↗

The importance of negative superhelicity in inducing the formation of G-quadruplex and i-motif structures in the c-Myc promoter: implications for drug targeting and control of gene expression.Semantic proximity Read ↗

Drosophila melanogaster topoisomerase IIIalpha preferentially relaxes a positively or negatively supercoiled bubble substrate and is essential during development.Semantic proximity Read ↗

DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic functionSemantic proximity Read ↗

Genome-wide DNA supercoiling arises from transcription and SMC activity and mediates transcriptional negative feedbackSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.