Article
Detection of the nt230[del4] MDR1 mutation in dogs by a fluorogenic 5' nuclease TaqMan allelic discrimination method.
Veterinary journal (London, England : 1997) - 1 Sept 2010
Klintzsch Stefanie, Meerkamp Kerstin, Döring Barbara, Geyer Joachim
Abstract excerpt
For detection of the nt230[del4] MDR1 mutation, a 4-bp deletion in the canine MDR1 (ABCB1) gene, a TaqMan allelic discrimination assay was designed that allows for MDR1 genotyping without post-PCR processing. Directly after completion of the PCR amplification, the MDR1 genotype can be assigned based on selective fluorescence measurement. For primer selection the locus of a potential 265A>G single nucleotide...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.