Article
Mutation of the human neu protein facilitates down-modulation by monoclonal antibodies.
Oncogene - 1 Apr 1990
van Leeuwen F, van de Vijver M J, Lomans J, van Deemter L, Jenster G, Akiyama T, Yamamoto T, Nusse R
Abstract excerpt
Amplification and overexpression of the neu gene have been found in several human adenocarcinomas. We have obtained monoclonal antibodies to the human neu protein by immunizing a Balb/c mouse with a Balb/c cell line expressing the human neu gene by transfection. The monoclonal antibodies reacted...
Topics
- Animals
- Antibodies, Monoclonal
- Cell Adhesion
- Cell Division
- Cell Line
- Cells, Cultured
- Clone Cells
- Flow Cytometry
- Fluorescent Antibody Technique
- Gene Expression Regulation, Neoplastic
- Humans
- Mammary Neoplasms, Experimental
- Mice
- Mice, Inbred BALB C
- Mutation
- Plasmids
- Protein-Tyrosine Kinases
- Proto-Oncogene Proteins