Article
Variants of tissue-type plasminogen activator which display substantially enhanced stimulation by fibrin.
The Journal of biological chemistry - 4 Aug 1995
Tachias K, Madison E L
Abstract excerpt
Unlike most proteases, tissue-type plasminogen activator (t-PA) is secreted from cells as an active, single chain "proenzyme" whose catalytic efficiency is comparable with that of the corresponding mature, two-chain enzyme. We have previously suggested that the absence of the "zymogen triad" (Asp...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- Chromogenic Compounds
- Enzyme Activation
- Enzyme Precursors
- Fibrin
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Processing, Post-Translational
- Structure-Activity Relationship