Article
Computational design of Candida boidinii xylose reductase for altered cofactor specificity.
Protein science : a publication of the Protein Society - 1 Oct 2009
Khoury George A, Fazelinia Hossein, Chin Jonathan W, Pantazes Robert J, Cirino Patrick C, Maranas Costas D
Abstract excerpt
In this study we introduce a computationally-driven enzyme redesign workflow for altering cofactor specificity from NADPH to NADH. By compiling and comparing data from previous studies involving cofactor switching mutations, we show that their effect cannot be explained as straightforward changes in volume, hydrophobicity, charge, or BLOSUM62 scores of the residues populating the cofactor binding site. Instead,...
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