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Expanding the cofactor range of aldehyde reductase YqhD by mutating key amino acids.

Biotechnology letters - 25 May 2026

Wu Xiaohui, Hu Yinghan, Huang Yanzhe, Zhang Lingyun, Xue Yan, Cao Hailong, Wang Xueying, Zhou Yongjin J

Abstract excerpt

YqhD is a broad-substrate aldehyde reductase that natively prefers NADPH and is widely used in alcohol biosynthesis. However, insufficient NADPH supply and low cofactor regeneration efficiency limit its catalytic performance. To address this limitation, we engineered YqhD through structure-guided semi-rational design and obtained a mutant G37D with markedly improved dual-cofactor compatibility with NADPH and...

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PMID: 42185663
DOI: 10.1007/s10529-026-03747-z

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