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Article

Creating Flavin Reductase Variants with Thermostable and Solvent-Tolerant Properties by Rational-Design Engineering.

Chembiochem : a European journal of chemical biology - 15 May 2020

Maenpuen Somchart, Pongsupasa Vinutsada, Pensook Wiranee, Anuwan Piyanuch, Kraivisitkul Napatsorn, Pinthong Chatchadaporn, Phonbuppha Jittima, Luanloet Thikumporn, Wijma Hein J, Fraaije Marco W, Lawan Narin, Chaiyen Pimchai, Wongnate Thanyaporn

Abstract excerpt

We have employed computational approaches-FireProt and FRESCO-to predict thermostable variants of the reductase component (C1 ) of (4-hydroxyphenyl)acetate 3-hydroxylase. With the additional aid of experimental results, two C1 variants, A166L and A58P, were identified as thermotolerant enzymes, with thermostability improvements of 2.6-5.6 °C and increased catalytic efficiency of 2- to 3.5-fold. After heat...

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Identifiers

PMID: 31886941
DOI: 10.1002/cbic.201900737

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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