Article
Enhancement in catalytic activity of Aspergillus niger XynB by selective site-directed mutagenesis of active site amino acids.
Applied microbiology and biotechnology - 1 Jan 2018
Wu Xiuyun, Tian Zhennan, Jiang Xukai, Zhang Qun, Wang Lushan
Abstract excerpt
XynB from Aspergillus niger ATCC1015 (AnXynB) is a mesophilic glycoside hydrolase (GH) family 11 xylanase which holds great potentials in a wide variety of industrial applications. In the present study, the catalytic activity and stability of AnXynB were improved by a combination of computational and experimental approaches. Virtual mutation and molecular dynamics simulations indicated that the introduction of...
Topics
- Amino Acids
- Aspergillus niger
- Catalysis
- Catalytic Domain
- Endo-1,4-beta Xylanases
- Enzyme Stability
- Evolution, Molecular
- Kinetics
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Conformation
- beta-Glucosidase