Article
Loss of rRNA modifications in the decoding center of the ribosome impairs translation and strongly delays pre-rRNA processing.
RNA (New York, N.Y.) - 1 Sept 2009
Liang Xue-Hai, Liu Qing, Fournier Maurille J
Abstract excerpt
The ribosome decoding center is rich in modified rRNA nucleotides and little is known about their effects. Here, we examine the consequences of systematically deleting eight pseudouridine and 2'-O-methylation modifications in the yeast decoding center. Loss of most modifications individually has no apparent effect on cell growth. However, deletions of 2-3 modifications in the A- and P-site regions can cause (1)...
Topics
- Base Sequence
- Cell Proliferation
- Drug Resistance, Fungal
- Efficiency
- Models, Biological
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- Organisms, Genetically Modified
- Protein Biosynthesis
- RNA Precursors