Article
Suboptimal codon pairs trigger ribosome collisions and cellular quality control responses in tRNA modification mutants.
Nucleic acids research - 26 Nov 2025
Wu Jie, Eggers Cristian, Sin Olga, Koziej Łukasz, Mancilla Hector, Mollet Fabienne, Schöler Hans R, Drexler Hannes C A, Ranff Tristan, Fufezan Christian, Kraft Claudine, Glatt Sebastian, Bruder Jan M, Leidel Sebastian A
Abstract excerpt
Transfer RNA (tRNA) modifications tune translation rates and codon optimality, thereby optimizing co-translational protein folding. However, the mechanisms by which tRNA modifications modulate codon optimality and trigger phenotypes remain unclear. Here, we show that ribosomes stall at specific modification-dependent codon pairs in wobble uridine modification (U34) mutants. This triggers ribosome collisions and a...
Read the complete abstract on PubMedTopics
- Ribosomes
- RNA, Transfer
- Codon
- Protein Biosynthesis
- Mutation