Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Transposon-directed base-exchange mutagenesis (TDEM): a novel method for multiple-nucleotide substitutions within a target gene.

BioTechniques - 1 Jun 2009

Kim Yun Cheol, Lee Hui Sun, Yoon Sukjoon, Morrison Sherie L

Abstract excerpt

In this report we describe transposon-directed base-exchange mutagenesis (TDEM), an efficient and controllable method for introducing a mutation into a gene. Each round of TDEM can remove up to 11 base pairs from a randomly selected site within the target gene and replace them with any length of DNA of predetermined sequence. Therefore, the number of bases to be deleted and inserted can be independently regulated...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Pairing
Share to beta-Galactosidase
Share to DNA Transposable Elements

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Lac Operon

Share to Mutagenesis, Site-Directed

Share to Nucleotides

Share to Open Reading Frames

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 19594453
DOI: 10.2144/000113152

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Transposon-directed base-exchange mutagenesis (TDEM): a novel method for multiple-nucleotide substitutions within a target gene.PMID 19594453Read ↗

Introducing predetermined mutations throughout a target gene using TDEM (transposon-directed base-exchange mutagenesis).Semantic proximity Read ↗

Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling.Semantic proximity Read ↗

A site-directed mutagenesis method particularly useful for creating otherwise difficult-to-make mutants and alanine scanning.Semantic proximity Read ↗

Polishing the craft of genetic diversity creation in directed evolution.Semantic proximity Read ↗

Transposon Insertion Sequencing, a Global Measure of Gene Function.Semantic proximity Read ↗

Chemical and UV Mutagenesis.Semantic proximity Read ↗

Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis.Semantic proximity Read ↗

Multiplexed in-situ mutagenesis driven by a dCas12a-based dual-function base editor.Semantic proximity Read ↗

DNA gap repair in Escherichia coli for multiplex site-directed mutagenesis.Semantic proximity Read ↗

Creating recombination-activated genes and sequence-defined mutant libraries using transposons.Semantic proximity Read ↗

Transposon mutagenesis.Semantic proximity Read ↗

Development of potent in vivo mutagenesis plasmids with broad mutational spectra.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.