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Article

Introducing predetermined mutations throughout a target gene using TDEM (transposon-directed base-exchange mutagenesis).

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Kim Yun Cheol

Abstract excerpt

Transposon-directed base-exchange mutagenesis (TDEM) is an efficient and controllable method for introducing a mutation(s) into a gene. Each round of TDEM removes a predetermined number of bases (up to 11 base pairs) from a randomly selected site within the target gene and replaces them with any length of DNA of predetermined sequence. Therefore, the number of bases to be deleted and inserted can be precisely...

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PMID: 21125393
DOI: 10.1007/978-1-61737-967-3_17

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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