Article
Whole genome amplification of buccal cell DNA: genotyping concordance before and after multiple displacement amplification.
Clinical chemistry and laboratory medicine - 1 Jan 2005
Thompson Miles D, Bowen Raffick A R, Wong Betty Y L, Antal Joan, Liu Zhanqin, Yu Helen, Siminovitch Katherine, Kreiger Nancy, Rohan Tom E, Cole David E C
Abstract excerpt
While buccal cells provide an easily accessible source of genomic DNA, the amount extracted may be insufficient for many studies. Whole genome amplification (WGA) using multiple displacement amplification (MDA) may optimize buccal cell genomic DNA yield. We compared the usefulness, in epidemiological surveys, of DNA derived from buccal cells collected by alcohol mouthwash and amplified by WGA protocol and...
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