Article
Single cell polymerase chain reaction for preimplantation genetic diagnosis: methods, strategies, and limitations.
Methods in molecular medicine - 1 Jan 2007
Sermon Karen, De Rycke Martine
Abstract excerpt
We provide an overview of the methodology involved in single cell polymerase chain reaction (PCR), especially for single lymphocytes or cultured lymphoblasts and blastomeres. The first step toward single cell PCR is isolation of single cells; the protocols given can be carried out using basic instruments such as a stereomicroscope. We also describe the alkaline lysis method for cell lysis as well as the design...
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