Article
Comparison of yield and genotyping performance of multiple displacement amplification and OmniPlex whole genome amplified DNA generated from multiple DNA sources.
Human mutation - 1 Sept 2005
Bergen Andrew W, Haque Kashif A, Qi Ying, Beerman Michael B, Garcia-Closas Montserrat, Rothman Nathaniel, Chanock Stephen J
Abstract excerpt
The promise of whole genome amplification (WGA) is that genomic DNA (gDNA) quantity will not limit molecular genetic analyses. Multiple displacement amplification (MDA) and the OmniPlex PCR-based WGA protocols were evaluated using 4 and 5 ng of input gDNA from 60 gDNA samples from three tissue sources (mouthwash, buffy coat, and lymphoblast). WGA DNA (wgaDNA) yield and genotyping performance were evaluated using...
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