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Article

A label-free strategy for SNP detection with high fidelity and sensitivity based on ligation-rolling circle amplification and intercalating of methylene blue.

Biosensors & bioelectronics - 15 Jul 2009

Zhang Songbai, Wu Zaisheng, Shen Guoli, Yu Ruqin

Abstract excerpt

A new strategy for label-free and sensitive detection of single-nucleotide polymorphism (SNP) based on ligation-rolling circle amplification (L-RCA) and intercalating of methylene blue is developed in the present work. A circular template generated by ligation upon the recognition of a point mutation on DNA targets was amplified isothermally by the Phi29 DNA polymerase. The elongation products were hybridized...

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PMID: 19481921
DOI: 10.1016/j.bios.2009.03.012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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