Article
Ligation-rolling circle amplification combined with γ-cyclodextrin mediated stemless molecular beacon for sensitive and specific genotyping of single-nucleotide polymorphism.
Talanta - 1 Jul 2014
Zou Zhen, Qing Zhihe, He Xiaoxiao, Wang Kemin, He Dinggeng, Shi Hui, Yang Xue, Qing Taiping, Yang Xiaoxiao
Abstract excerpt
A novel approach for highly sensitive and selective genotyping of single-nucleotide polymorphism (SNP) has been developed based on ligation-rolling circle amplification (L-RCA) and stemless molecular beacon. In this approach, two tailored DNA probes were involved. The stemless molecular beacon, formed through the inclusion interactions of γ-cyclodextrin (γ-CD) and bis-pyrene labeled DNA fragment, was served as...
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