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Article

Rolling circle amplification combined with gold nanoparticle aggregates for highly sensitive identification of single-nucleotide polymorphisms.

Analytical chemistry - 1 Apr 2010

Li Jishan, Deng Ting, Chu Xia, Yang Ronghua, Jiang Jianhui, Shen Guoli, Yu Ruqin

Abstract excerpt

A highly sensitive and specific colorimetry-based rolling circle amplification (RCA) assay method for single-nucleotide polymorphism genotyping has been developed. A circular template is generated by ligation upon the recognition of a point mutation on DNA targets. An RCA amplification is then initiated using the circular template in the presence of Phi29 polymerase. The RCA product can be digested by a...

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DNA Probes
Genotype
Gold
Metal Nanoparticles
Nucleic Acid Amplification Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Single Nucleotide

Spectrophotometry, Ultraviolet

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PMID: 20192245
DOI: 10.1021/ac100336n

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