Article
Depletion of phosphatidylcholine affects endoplasmic reticulum morphology and protein traffic at the Golgi complex.
Journal of lipid research - 1 Nov 2009
Testerink Nicole, van der Sanden Michiel H M, Houweling Martin, Helms J Bernd, Vaandrager Arie B
Abstract excerpt
The mutant Chinese hamster ovary cell line MT58 contains a thermosensitive mutation in CTP:phosphocholine cytidylyltransferase, the regulatory enzyme in the CDP-choline pathway. As a result, MT58 cells have a 50% decrease in their phosphatidylcholine (PC) level within 24 h when cultured at the no...
Topics
- Animals
- CHO Cells
- Cell Membrane
- Cell Proliferation
- Cell Survival
- Choline-Phosphate Cytidylyltransferase
- Cricetinae
- Cricetulus
- Endoplasmic Reticulum
- Fluorescence Recovery After Photobleaching
- Golgi Apparatus
- Green Fluorescent Proteins
- Lysophosphatidylcholines
- Membrane Glycoproteins
- Microscopy, Confocal
- Microscopy, Immunoelectron
- Mutation
- Oleic Acid