Occurrence of Ki-ras and p53 mutations in primary colorectal tumors.

Abstract excerpt

Twenty-four colorectal tumors and their paired mucosa were examined for allele loss on chromosomes 5 and 17, using polymerase chain reaction-amplified DNA, and for mutations in the Ki-ras and p53 genes. In addition, 19 benign polyps were analysed for mutations in the p53 gene. RFLP analysis of the long arm of chromosome 5 indicated an allele loss frequency of 47% for malignant tumors, a value somewhat higher than...

Topics

• Blotting, Northern
• Blotting, Southern
• Chromosomes, Human, Pair 17
• Chromosomes, Human, Pair 5
• Codon
• Colorectal Neoplasms
• DNA Mutational Analysis
• Genes, p53
• Genes, ras
• Humans
• Mutation