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Article

High sensitivity of both sequencing and real-time PCR analysis of KRAS mutations in colorectal cancer tissue.

Journal of cellular and molecular medicine - 1 Aug 2010

Tol Jolien, Dijkstra Jeroen R, Vink-Börger Marianne E, Nagtegaal Iris D, Punt Cornelis J A, Van Krieken Johan H J M, Ligtenberg Marjolijn J L

Abstract excerpt

The KRAS mutation status predicts the outcome of treatment with epidermal growth factor receptor targeted agents, and therefore the testing for KRAS mutations has become an important diagnostic procedure. To optimize the quality of this test, we compared the results of the two most commonly used KRAS mutation tests, cycle sequencing and a real-time PCR-based assay, in DNA extracted from formalin-fixed...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 19453520
DOI: 10.1111/j.1582-4934.2009.00788.x
PMCID: PMC3823003

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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High sensitivity of both sequencing and real-time PCR analysis of KRAS mutations in colorectal cancer tissue.PMID 19453520Read ↗

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454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samplesSemantic proximity Read ↗

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