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Article

High sensitivity of both sequencing and real-time PCR analysis of KRAS mutations in colorectal cancer tissue.

Journal of cellular and molecular medicine - 1 Aug 2010

Tol Jolien, Dijkstra Jeroen R, Vink-Börger Marianne E, Nagtegaal Iris D, Punt Cornelis J A, Van Krieken Johan H J M, Ligtenberg Marjolijn J L

Abstract excerpt

The KRAS mutation status predicts the outcome of treatment with epidermal growth factor receptor targeted agents, and therefore the testing for KRAS mutations has become an important diagnostic procedure. To optimize the quality of this test, we compared the results of the two most commonly used KRAS mutation tests, cycle sequencing and a real-time PCR-based assay, in DNA extracted from formalin-fixed...

Topics

Colorectal Neoplasms
DNA Mutational Analysis
DNA, Neoplasm
Gene Frequency
Genes, ras
Genotype
Humans
Mutation
Polymerase Chain Reaction
Reproducibility of Results

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sensitivity and Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 19453520
DOI: 10.1111/j.1582-4934.2009.00788.x
PMCID: PMC3823003

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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