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Article

Sanger sequencing in routine KRAS testing: a review of 1720 cases from a pathologist's perspective.

Journal of clinical pathology - 1 Oct 2012

Malapelle Umberto, Bellevicine Claudio, Salatiello Maria, de Luca Caterina, Rispo Elisabetta, Riccio Palmira, Sparano Lucianna, De Stefano Alfonso, Carlomagno Chiara, Maiello Francesco Maria, Vita Giulia, Nappi Oscar, Troncone Giancarlo

Abstract excerpt

BACKGROUND: Sanger sequencing (SS) of PCR products is still the most frequent method to test colorectal cancer for KRAS mutations in routine practice. METHODS: An audit of SS on 1720 routine cases was carried out, taking into account age, gender, specimen type (resection vs biopsies), tumour site (primary vs metastasis), tumour stage, neoplastic cells abundance (>30% vs <30%) and fixation type (buffered formalin...

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Identifiers

PMID: 22872705
DOI: 10.1136/jclinpath-2012-200773
PMCID: PMC3461636

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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