Article
KRAS gene mutation in a series of unselected colorectal carcinoma patients with prognostic morphological correlations: a pyrosequencing method improved by nested PCR.
Experimental and molecular pathology - 1 Jun 2015
de Macêdo Mariana Petaccia, de Melo Fernanda Machado, Lisboa Bianca Cristina Garcia, Andrade Louise D Brot, de Souza Begnami Maria Dirlei Ferreira, Junior Samuel Aguiar, Ribeiro Heber Salvador de Castro, Soares Fernando Augusto, Carraro Dirce Maria, da Cunha Isabela Werneck
Abstract excerpt
INTRODUCTION: Inhibition of EGFR is a strategy for treating metastatic colorectal cancer (CRC) patients. KRAS sequencing is mandatory for selecting wild-type tumor patients who might benefit from this treatment. DNA from formalin-fixed paraffin-embedded (FFPE) tissues is commonly used for routine clinical detection of mutations, and its amplification succeeds only when all preanalytical histological processes...
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