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Article

Expression of bacterial Rho factor in yeast identifies new factors involved in the functional interplay between transcription and mRNP biogenesis.

Molecular and cellular biology - 1 Aug 2009

Mosrin-Huaman Christine, Honorine Romy, Rahmouni A Rachid

Abstract excerpt

In eukaryotic cells, the nascent pre-mRNA molecule is coated sequentially with a large set of processing and binding proteins that mediate its transformation into an export-competent ribonucleoprotein particle (mRNP) that is ready for translation in the cytoplasm. We have implemented an original assay that monitors the dynamic interplay between transcription and mRNP biogenesis and that allows the screening for...

Topics

Blotting, Northern
Blotting, Western
Cell Nucleus
Escherichia coli Proteins
Gene Expression
Mutation
Plasmids
RNA Polymerase II
Rho Factor
Ribonucleoproteins
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transcription, Genetic

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Identifiers

PMID: 19451224
DOI: 10.1128/MCB.00272-09
PMCID: PMC2715799

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